The objective of the present study was to determine the number of spermatozoa obtained from the different part of the oviduct and uterine horn after intrauterine insemination (IUI) compared with conventional artificial insemination (AI). Twelve crossbred (Landrace x Yorkshire) multiparous sows were used in this study. All sows were examined for estrus every 6 h using back pressure test with the boar and were detected for the ovulation time using transrectal real time B-mode ultrasound. An artificial insemination was carried out using one boar at 8-10 h before the expected ovulation during the second estrus. The sows were divided in two groups, group I (n=6) was inseminated with conventional artificial insemination method with 3 x 10[superscript 9] spermatozoa in 100 ml extended semen and group II (n=6) was inseminated with intrauterine insemination technique with 1 x 10[superscript 9] spermatozoa in 50 ml extended semen. Twenty four hours after insemination, all sows were ovario-hysterectomized, oviducts and uterine horns were removed and divided into different seven parts as cranial, middle and caudal uterine horn, UTJ, cranial and caudal isthmus and ampulla. All parts were flushed and determined the number of spermatozoa by using haemocytometer. The results showed that there were not significant difference in the number of spermatozoa in both side of oviducts and uterine horns of group I and II (p>0.05). The numbers of flushed spermatozoa in UTJ and caudal isthmus for group I and II were 142,500; 131,167; 1,411 and 1,280, respectively. The proportion of sperm in all parts between group I and group II were not significantly different (p>0.05).