การเปรียบเทียบรูปแบบการดื้อยาและแบบแผนดีเอ็นเอของเชื้อเอ็นเตอโรคอคคัยที่ดื้อยาแวนโคมัยซินที่แยกได้จากสุนัข แมวและเจ้าของ / กนกดล สิริวัฒนชัย = Comparison of antimicrobial resistance and DNA patterns of vancomycin-resistant enterococci isolated from dogs cats and owners / Kanokdon Siriwattanachai
Epidemiology of vancomycin-resistant Enterococci (VRE) between companion dogs and cats and human has not been studied. Five-hundred and thirty fecal samples of dogs and cats; which were randomly collected from companion dogs and cats at Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University, Thailand; were screened for VRE by selective media contained 6 microgram of vancomycin per mL. VRE were detected 61 isolates (11.5 %) from companion dogs and cats which were classified as Enterococcus faecium 16 isolates (26.2 %), E. faecalis 3 isolates (4.9 %), E. gallinarum 37 isolates (60.7 %), and E. casseliflavus 5 isolates (8.2 %). Of 61 owners of VRE colonized dogs and cats, 21 persons (from 16 households of VRE colonized dogs) participated in this study. Fifteen persons or 71.4 % (from 11 households) were found VRE in their fecal samples and were classified as E. faecium 6 isolates (40%) and E. gallinarum 9 isolates (60 %). Antimicrobial susceptibility test had been performed by using agar dilution method (vancomycin : VN, ampicillin : AP, erythromycin : ET, tylosin : TS, gentamicin : GM, chloramphenicol : CHPC, nitrofurantoin : NF and tetracycline : TC) and E-test (teicoplanin : TP). Among 61 VRE isolated from companion dogs and cats, 16 isolates E. faecium were resistant to VN, AP, ET, TS, GM, NF, and TC. Three isolates E. faecalis were resistant to ET, TS, GM, and CHPC. Thirty-seven isolates E. gallinarum were resistant to AP, ET, TS, GM, CHPC, and TC, while 5 isolates of E. casseliflavus was resistant to ET and GM. Of 15 VRE isolated from owners of dogs 6 isolates were E. faecium which were resistant to AP, ET, TS, GM, and TC, while 9 isolates E. gallinarum were resistant to all tested antibiotics except VN, TP, CHPC, and NF. By comparison of antimicrobial resistance patterns between 11 VRE isolated from dogs and their owners, four VRE isolated dogs and their owners revealed similar patterns of antimicrobial susceptibility. Detection of van gene of VRE by technique of PCR, vanC1 was found in 37 isolates of E. gallinarum (100 %), and vanC2/C3 was found in 5 isolates of E. casseliflavus(100 %) which they were isolated from dogs and cats. Whereas, vanC1 was found in 9 isolates of E. gallinarum (100 %) which they were isolated from owners. Comparison of DNA patterns of VRE isolated from dogs and owners by PFGE revealed that their VRE clones were different. Therefore, this study implied that VRE colonized in companion dogs might not be the epidemiological significance of transmitting to human.