ผลของสารสกัดกวาวเครือแดงต่อหลอดเลือดดำที่แยกจากสายสะดือมนุษย์ / รุจิเรข บุญกาพิมพ์ = Effect of the extract from Butea superba on isolated human umbilical vein / Rujirek Boongapim
Ethanol extract of Butea superba was prepared. Thin layer chromatography (TLC) characterizing pattern of compounds in the ethanol extract of Butea superba was performed to obtain TLC fingerprint. To assess the vasodilating effect and mode of action of Butea superba extract (BSE), isolated human umbilical vein was used. The effect was first studied whether its action was endothelium mediated. The strips of isolated vein with or without endothelium were induced contraction with KCl. Nitric oxide synthase (NOS) inhibitor, Cyclooxygenase (COX) inhibitor and Potassium channel blocking agent are used in separated experiments with the same procedure as mentioned above in order to confirm the role of these mediators in producing vasodilating effect of Butea superba extract. The results showed inhbibitory effect of Butea superba extract on KCl (high concenteation) and histamine - induced vascular contraction.The inhibitory effect of the extract showed time and concentration - dependent manners. Moreover, the inhibitory effect of BSE required endothelium function. This study indicated that the vasodilating effects of BSE involved the EDRFs and EDHFs. There are at least 3 pathways of EDRFs and EDHFs such as Nitric oxide (NO), Prostacyclin (PGI[subscript 2]) and Endothelium-Derived Hyperpolarizing Factors (EDHFs) involved in the inhibitory action of BSE. Whereas Butea superba extract was unable to inhibit the 5-HT - induced vascular contraction.
