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TitleEnzyme functionality : design, engineering, and screening / edited by Allan Svendsen
Imprint New York : Marcel Dekker, 2004
Descript xix, 667 p. : ill. ; 24 cm


1. Concepts for Protein Engineering -- 2. Sequence Families and Modular Organization of Carbohydrate-Active Enzymes -- 3. Analyzing Three-Dimensional Structures of Variant Enzymes -- 4. Quantitative Modeling of Lipase Enantioselectivity -- 5. Rational Redesign of Haloalkane Dehalogenases Guided by Comparative Binding Energy Analysis -- 6. Computer Simulations: A Tool for Investigating the Function of Complex Biological Macromolecules -- 7. Calculations of Ionization Equilibria in Proteins -- 8. Modeling and Optimization of Directed Evolution Protocols -- 9. Rational Redesign of Enzymes -- 10. Details in the Reaction Mechanism of Chitinases -- 11. Kinetic Evolution to the Catalytic Core of the Bacterial Phosphotriesterase -- 12. Protein Engineering of PQQ Glucose Dehydrogenase -- 13. The Proline Rule: A Concept for Engineering Protein Stability -- 14. Homing Endonucleases: Tools and Targets for Protein Engineering -- 15. Evolutionary Methods for Protein Engineering -- 16. Directed Evolution by Random Mutagenesis: A Critical Evaluation -- 17. Enzyme Engineering by Phage Display -- 18. In Vivo Gene Shuffling in Yeast: A Fast and Easy Method for Directed Evolution of Enzymes -- 19. Effective DNA Shuffling Methods for Enzyme Evolution -- 20. Exploring the Functional Space of Combinatorial Mutant Libraries for the Directed Evolution of Novel Enzyme Activities -- 21. Modifying the Character of an Enzyme by Producing Chimeric Enzymes: Chimeric [beta]-glucosidases as an Illustration -- 22. Assay Systems for Screening or Selection of Biocatalysts -- 23. Screening of Enzyme Variants for Thermostability -- 24. Combinatorial Mutagenesis Algorithms, Digital Imaging Spectroscopy, and Solid-Phase Assays for Directed Evolution -- 25. Screen Automation and Robotics -- 26. Screening for Enantioselective Enzymes -- 27. Enzyme Engineering by Microbial Cell Surface Display -- 28. Overexpression and Secretion of Biocatalysts in Pseudomonas -- 29. Analysis of Catalytic and Structural Stability of Native and Covalently Modified Enzymes

Enzymes -- Design Protein engineering

Science LibraryTP248.65.E59 E61s 2004CHECK SHELVES

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