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TitleE. coli gene expression protocols / edited by Peter E. Vaillancourt
Imprint Totawa, N.J. : Humana Press, c2003
Descript xi, 347 p. : ill. ; 24 cm


1. Cold-Inducible Promoters for Heterologous Protein Expression -- 2. Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells -- 3. A Dual-Expression Vector Allowing Expression in E. coli and P. pastoris, Including New Modifications -- 4. Purification of Recombinant Proteins from E. coli by Engineered Inteins -- 5. Calmodulin as an Affinity Purification Tag -- 6. Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification -- 7. Maltose-Binding Protein as a Solubility Enhancer -- 8. Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli -- 9. Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli -- 10. Assessment of Protein Folding/Solubility in Live Cells -- 11. Improving Heterologous Protein Folding via Molecular Chaperone and Foldase Co-Expression -- 12. High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins -- 13. Co-Expression of Proteins in E. coli Using Dual Expression Vectors -- 14. Small-Molecule Affinity-Based Matrices for Rapid Protein Purification -- 15. Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli -- 16. Screening Peptide/Protein Libraries Fused to the [lambda] Repressor DNA-Binding Domain in E. coli Cells -- 17. Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System -- 18. Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions -- 19. Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli -- 20. Hyperphage: Improving Antibody Presentation in Phage Display -- 21. Combinatorial Biosynthesis of Novel Carotenoids in E. coli -- 22. Using Transcriptional-Based Systems for In Vivo Enzyme Screening -- 23. Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis

Gene expression -- Laboratory manuals Escherichia coli -- Genetics -- Laboratory manuals Escherichia coli -- genetics -- Laboratory Manuals Gene Expression -- Laboratory Manuals

Science LibraryQH450 E11v 2003CHECK SHELVES

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