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TitleProtein sequencing protocols / edited by Bryan John Smith
Imprint Totowa, N.J. : Humana Press, c2003
Edition 2nd ed
Descript xii, 493 p. : ill. ; 23 cm


1. Strategies for Handling Polypeptides on a Micro-Scale -- 2. SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing -- 3. Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization -- 4. Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization -- 5. Enzymatic Cleavage of Proteins -- 6. Chemical Cleavage of Polypeptides -- 7. Enzymatic Preparation and Isolation of Glycopeptides -- 8. Analytical and Micropreparative Capillary Electrophoresis of Peptides -- 9. High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues -- 10. Hydrolysis of Samples for Amino Acid Analysis -- 11. Amino Acid Analysis: Precolumn Derivatization Methods -- 12. Post Column Amino Acid Analysis -- 13. Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples -- 14. Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection -- 15. D-Amino Acid Analysis -- 16. Validation of Amino Acid Analysis Methods -- 17. Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins -- 18. Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- 19. Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes -- 20. Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis -- 21. Identification of PTH-Amino Acids by HPLC -- 22. Protein Sequencer Maintenance and Troubleshooting -- 23. N-Terminal Protein Sequencing for Special Applications -- 24. Identification of Phosphorylation Sites by Edman Degradation -- 25. Validation of Protein Sequencing in a Regulated Laboratory -- 26. Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method -- 27. Chemical Modifications of Proteins as an Aid to Sequence Analysis -- 28. Deblocking of N-Terminally Modified Proteins -- 29. Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid -- 30. Identification of Sites of Glycosylation -- 31. Analysis of Sites of Protein Phosphorylation -- 32. Quantitation and Location of Disulfide Bonds in Proteins -- 33. Getting the Most from Your Protein Sequence -- App.1: Letter Codes, Structures, Masses, and Derivatives of Amino Acids -- App.2: Protein Consensus Sequence Motifs

Nucleotide sequence -- Laboratory manuals Sequence Analysis Protein -- Laboratory Manuals Proteins -- analysis -- Laboratory Manuals

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