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TitlePCR protocols / edited by John M.S. Bartlett and David Stirling
Imprint Totowa, N.J. : Humana Press, c2003
Edition 2nd ed
Descript xvii, 545 p. : ill. ; 26 cm

CONTENT

1. A Short History of the Polymerase Chain Reaction -- 2. PCR Patent Issues -- 3. Equipping and Establishing a PCR Laboratory -- 4. Quality Control in PCR -- 5. Extraction of Nucleic Acid Templates -- 6. Extraction of DNA from Whole Blood -- 7. DNA Extraction from Tissue -- 8. Extraction of DNA from Microdissected Archival Tissues -- 9. RNA Extraction from Blood -- 10. RNA Extraction from Frozen Tissue -- 11. RNA Extraction from Tissue Sections -- 12. Dual DNA/RNA Extraction -- 13. DNA Extraction from Fungi, Yeast, and Bacteria -- 14. Isolation of RNA Viruses from Biological Materials -- 15. Extraction of Ancient DNA -- 16. DNA Extraction from Plasma and Seru -- 17. Technical Notes for the Detection of Nucleic Acids -- 18. Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels -- 19. PCR Primer Design -- 20. Optimization of Polymerase Chain Reactions -- 21. Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine - Cystine Content: Amplification of the Intron 22 Inversion of the FVIII Gene -- 22. Rapid Amplification of cDNA Ends -- 23. Randomly Amplified Polymorphic DNA Fingerprinting: The Basics -- 24. Microsphere-Based Single Nucleotide Polymorphism Genotyping -- 25. Ligase Chain Reaction -- 26. Nested RT-PCR in a Single Closed Tube -- 27. Direct PCR from Serum: Application to Viral Genome Detection -- 28. Long PCR Amplification of Large Fragments of Viral Genomes: A Technical Overview -- 29. Long PCR Methodology -- 30. Qualitative and Quantitative PCR: A Technical Overview -- 31. Ultrasensitive PCR Detection of Tumor Cells in Myeloma -- 32. Ultrasensitive Quantitative PCR to Detect RNA Viruses -- 33. Quantitative PCR for cAMP RI Alpha mRNA: Use of Site-Directed Mutation and PCR Mimics -- 34. Quantitation of Multiple RNA Species -- 35. Differential Display: A Technical Overview --


Polymerase chain reaction -- Laboratory manuals

LOCATIONCALL#STATUS
Science LibraryQH506 P348b 2003CHECK SHELVES

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