การศึกษาเปรียบเทียบผลของวัสดุเอ็มทีเอกับวัสดุกลาสไอโอโนเมอร์ซีเมนต์ชนิดดัดแปลงด้วยเรซินต่อเซลล์เพาะเลี้ยงจากเอ็นยึดปริทันต์ของมนุษย์ / อุทัยวรรณ อารยะตระกูลลิขิต = The comparative effect of MTA and RMGIC on human periodontal ligament cells, in vitro / Uthaiwan Arayatrakoollikit
The purpose of this in vitro study was to compare the effect of MTA with RMGIC on human periodontal ligament cells. Proliferation assay was performed by using colorimetric assay at 48 and 72 hours and alkaline phosphatase activity was determined by using biochemical assay at 24, 72 and 120 hours. Both materials were fabricated and eluated with serum free medium for 24 hours. Cells were treated with different concentrations of eluated materials per fresh medium with the ratio of 1:0, 1:1, 1:2, 1:4, and 1:8. One way ANOVA and Tukey's test (p<0.05) were used to detect differences between the treatment and the control groups. The proliferation assay showed that all concentrations of RMGIC and 1:8 concentration of MTA significantly stimulated PDL cell proliferation at 48 and 72 hours. All concentrations of RMGIC significantly decreased alkaline phosphatase activity at 72 and 120 hours. However, only 1:8 concentration of MTA significantly decreased alkaline phosphatase activity at 24, 72 and 120 hours. Therefore, the result of this study showed that RMGIC has more favorable cellular response than MTA in term of cellular proliferation. In contrast , MTA has more favorable cellular response than RMGIC in term of alkaline phosphatase activity.