ผลของน้ำมันระเหยจากใบของต้นหัสคุณใหญ่ต่อการหดตัวของกล้ามเนื้อเรียบ / ประภัค ศรีกิติกุลชัย = Effects of volatile oil from the leaves of clausena anisata hook on smooth muscle contraction / Parpak Srikiticoolchai
Preliminary study of the pharmacological action of volatile oil from the leaves of clausena anisata Hook. was carried out in various smooth muscle preparations. Cumulative doses of the essential oil (5x10[superscript -5]-3.2x10[superscript -3]%v/v) stimulated the contractile response of all smooth muscle preparation. The highest stimulation was found in isolated rat aorta (47.03%, EC[subscript 50] = 1.28x10[superscript -2]%). The others were guinea-pig ileum (39.40%: EC[subscript 50] = 9.60x10[superscript -3]%) rat fundus (26.19%: EC[subscript 50] = 8.19x10[superscript -1]%) guinea-pig trachea (15.78%) and rabbit jejunum (4.99%). These spasmodic effects were investigated through autonomic receptors. The result demonstrated that atropine was not able to attenuate the stimulation effect of the essential oil on the isolated rabbit jejunum and guinea-pig ileum while the inhibitory effects of atropine (1x10[superscript -7] and 1x10[superscript -6]M) were prominently found in the contraction induced by the essential oil on rat fundus. Relaxation effect was insignificantly shown in guinea-pig trachea after exposure to atropine. Sympathetic mechanism of the essential oil was confirmed in rat aorta since prazosin (1x10[superscript -7]M) reduced the contractile response produced by the essential oil significantly. The source of calcium ion responsible for the essential oil-induced aortic contraction was elucidated. It was shown that verapamil and calcium free Krebs-Henseleit medium could suppress the aortic contraction evoked by the essential oil. All these results could be concluded that the essential oil from the leaves of clausena anisata possessed smooth muscle stimulation effect partly through sympathetic and parasympathetic receptors.
