To study a possibility of reusing various enzymes in cotton scouring process. Enzymes were reused both with and without a pH adjustment of the enzyme solution before each reuse. Four enzymes containing pectinase, lipase, protease, and cellulase were used for this task. Greige cotton fabric was scoured in various enzyme solutions at conditions suitable for each enzyme. Then the solutions were reused for scouring other greige fabrics. The experiment was carried out this way until the solutions showed inefficient scouring power. All scoured fabrics were tested for water absorbency, dyeability, whiteness, bursting strength, pectin content and weight loss. It was found that the enzyme solution could not be reused sufficiently without a pH adjustment of the enzyme solution before reuse. While reusing the pH adjusting enzyme solution showed an adequate scouring. Pectinase enzyme could be reused twice while the other enzymes (lipase, protease, and cellulase) could be reused only once for fabric scouring. Results also indicated that the reused enzyme showed approximately the same scouring power as the first used enzyme.