Fusarlum species are commonly found in various crops, especially cereals. Fusarium mycotoxins such as fumonisin, moniliformin, fusaric acid, fusarin and trichothecenes cause mycotoxicosis in humans and animals. The objectives of this study were to : isolate and identify Fusarium spp. from domestic maize; induce suitable laboratory conditions for high yield mycotoxin production and to study toxicopathology เท broiler fed with contaminated maize. The experiment was conducted by collecting 122 maize samples were from various part of Thailand. ELISA test kit ( Veratox™) was used to detect fumonisin, T-2 toxin, ochratoxin and aflatoxin. The maize samples were also used for isolate fungi on PCNB-peptone agar, potato dextrose agar (PDA) and cornmeal agar dilution plate. Fumonisin and T-2 toxin were detected from all Fusarium strains. Eight Fusarium strains from maize kernels producing over 4,000 ppb fumonisin were identified by measuring growth rate and spore formation on PDA 1 potato sucrose agar, corn leaf agar and corn seed agar and observing morphological characterestics under stereo- and compound microscopes. The strains were identified as Fusarium monillforme Sheldon and all produced fumonisin and T-2 toxin. F. monlllforme no. 4262, which produced the highest fumonisin and T-2 toxin content, was cultivated on sterile kernels for 35 days. The culture materials were mixed in feed for 166, day-old chicks for 25 days. The experiment was divided into control and treatment groups. The result