The Leiolepis belliana belliana (Gray), a common reptile found in the all regions including the islands of Thailand, has a very important role in the ecosystem and serves as food resource for human beings. This research was therefore initiated. These animal samples were collected from Sa-maesarn Island, Khram Island, Rin Island and Phai Island at Sataheep District and some animal samples were collected from Khaokheaw and Khaochompoo Sriracha District, Chonburi Province. Chromosomes from lymphocyte cultures were studied by conventional staining and G-banding technique. The result showed that all of these animals have the same chromosome number 2n=36, relatively identical in shape and size. The chromosome complements of L.b. belliana composed of 12 macrochromosomes and 24 microchromosomes. Six pairs of macrochromosomes were classified as follows: five pairs were of metacentric-type and the other were of submetacentric-type. A secondary constriction was found on the long arm of the first chromosome pair. The microchromosomes were too small to be classified. According to the result obtained from G-banding technique, the specimens from four types of population have the same pattern. This karyotypic information will be useful for further genetic diversity study on the same lizard from other regions of Thailand.