Pseudomonas aeruginosa produced a high amount of lipase in 5-liter fermenter. Culture medium contains (NH4)2SO4 0.13% (w/v), Fructose 2% (w/v), K2HPO4 0.09% (w/v), KH2PO4 0.06% (w/v), MgSO4.7H2O 0.02% (w/v) and yeast extract 0.01% (w/v) in total volume of 4 liter. The optimal conditions for culturing were as follows: inoculum size at 0.5% (v/v) of the culture volume, aeration rate at 1.0 vvm, agitation speed of 250 rpm, temperature at 37ํC and control pH at 7.0. This strain can produce the high amount of lipase 1,476.50 unit/ml. at 48 hours. The enzyme was filtrated by ultrafiltration and made in powder form by lyophilization. The specific activity of powder enzyme was 98.04 unit/mg powder. The enzymatic scouring of cotton fabrics treated by lipase from Pseudomonas aeruginosa, protease from Bacillus subtilis TISTR25 and cellulase from Trichoderma reesei made an adequate absorbency of cotton fabrics.
