ผลของโซเดียมคลอไรด์ที่มีต่อการเจริญเติบโตและการสะสมโพรลีนโซเดียมไอออนและคลอไรด์ไออนในถั่วเหลือง Glycine max (L.) Merrill / พรศักดิ์ ภักดีวราภรณ์ = Effects of sodium chloride on growth and accumulation of proline, sodium ion and chloride ion in soybeans Glycine max (L.) Merrill / Pornsak Pakdeevaraporn
The effects of sodium chloride on growth and proline, sodium ion and chloride ion accumulation were determined in three soybean (Glycine max (L.) Merrill) cultivars; SJ.5, CM.60 and ST.2. Sodium chloride was added to the Hoagland nutrient solution at the concentration of 20, 40 and 80 mM. The plants grown in the Hoagland nutrient solution was used as a control for all experiments. The soybean plants were harvested every fifteen days for two months to determine growth and proline, sodium ion and chloride ion accumulation during salt-stress treatments. Every sodium chloride treatment resulted in the significant reduction of vegetative and reproductive growth in all three soybean cultivars. Growth response suggested that ST.2 was the most salt-sensitive among these three cultivars. This conclusion was also supported with the reduction of leaf area ratio of ST.2, while salt-stress showed no significant effects on leaf ratio in SJ.5 and CM.60. The highest level of proline accumulation was detected in ST.2 leaves, which suggested the consequence of protein damage. All three cultivars showed the similar results for sodium and chloride ion accumulation. The highest level of both ion species was detected in roots and the lowest level was detected in pods, and the older leaves showed the higher level of ion accumulation.
