การผลิตไซแลนเนสจาก Trichoderma reesei โดยใช้วัสดุเหลือใช้จากการเกษตร / เนริสา คุณประทุม = Production of xylanase from Trichoderma reesei using agricultural wastes / Narisa Kunpratum
richoderma reesei Rut C-30 was cultivated in production medium at pH 5 temperture 30 ํC, using agricultural wastes including wheat bran, rice bran, sugarcane bagasse and peanut hulls as the sole carbon sources. Wheat bran gave highest xylanase activity (23.931 IU/ml). Rice bran gave highest cellulase activity (0.656 U/ml). Chemical analysis of agricultural wastes component were accomplished. Wheat bran contained the highest hemicellulose or xylan, which contained 26.10 gram per dry weight. Peanut hulls contained highest cellulose (35.13 gram per dry weigh). The presence of relatively high hemicellulose content in the wheat bran helped support the production of xylanase. In the attempt to find suitable concentration of wheat bran, the substrate at 5% level gave the highest activity (27.946 U/ml). Corn steep liquor 0.1% was the best nitrogen source. Xylan, xylose and beta-methyl-xyloside for xylanase production were investigated as the inducers. Wheat bran plus xylan slightly induced xylanase activity. Wheat bran plus 0.2 g/l of xylose could induce 1.85 folds of xylanase production. Beta-methyl-xyloside induced xylanase production 5.32 folds.
