การใช้สารยับยั้งการสร้างหลอดเลือดในหนูทดลองที่ได้รับการปลูกถ่ายเซลล์มะเร็งตับจากมนุษย์ / ประสาร ขจรรัตนเดช = The use of antiangiogenesis agent in a human-hepatocellular-carcinoma implanted nude mice model / Prasan Kachonrattanadet
Objective To determine the objective response of antiangiogenesis agent for treatment of hepatocellular carcinoma cell line implanted in nude mice Method The human hepatocellular carcinoma cell lines, HEP G2 and S 109, were cultured in media. The 7 week-old nude mice were operated under anesthesia and dorsal skin chambers were placed on the backs of all nude mice subcutaneously. The HEP G2 and S 109 cell lines were implanted in all chambers with the amount of 80,000 cells in each chamber. All nude mice were maintained in pathogen-limited condition in the appropriate environment. Then the nude mice were separated into two groups, experimental and controlled groups, with three mice in each. The antiangiogenesis agent, marimastat, were diluted with water to the concentration of 1 mg/ml. The experimental nude mice were fed by oral gavage with the dosage of 10 mg/kg/day of this agent twice a day. The controlled nude mice were also fed with water in the same volume. After 7 days each mice were examined under anesthesia by intravital fluorescent microscopy with video recording. Microvessel densities and colony count per unit area were calculated by image program. The results from both groups were compared for hypothesis testing using unpaired t-test method. Results The MMP inhibitor, marimastat, exerted a statistical significant therapeutic effect, reducing the percentage of microvascular area per one unit area and the percentage of area of cancer cells colony per one unit area. No sign of any side effect was found in the experimental group. Conclusion: The nude mice with implanted hepatocellular carcinoma cell lines were effectively treated with antiangiogenesis agent.
