Calli of rice (Oryza sativa L.) cv. RD23 were obtained from mature embryos by culturing mature embryos on five different media. Only Nitiwatana medium (1995) was selected for futher experiments. All calli obtained from this medium were of embryogenic type and bigger than other media. The studies on genetic markers for salt tolerant was carried out using selected salt tolerant lines (RD23TC7 and RD23TC28) obtained from somaclones. These were done by using DAF and RAPD techniques with UBC and CU primers respectively. The results obtained when using UBC 457 (CGACGCCCTG) showed one DNA band in RD23TC7 which differed from the original line, this also obtained when using CU2 (CCACAGCAGT) primer on the same line. On the contrary. when using CU1 (GTTTCGCTCC) primer the band appeared on both RD23TC7 and RD23TC28 but not original line. When using the CU primers, the target DNA band on some of the offspring between may be used as genetic marker for salt tolerant rice lines
