การศึกษาแฮปโพลไทป์ของกลุ่มยีนบีตาโกลบินและรูปแบบการขาดหายไปของยีนแอลฟาโกลบินในชนกลุ่มชาวน้ำ จังหวัดภูเก็ต / นันทวรรณ ชื่นชมคุณาธร = Study of beta-globin gene cluster haplotypes and gene deletion type of alpha-globin gene in Chaonam population in Phuket province / Nuntawan Chuenchomkunatorn
Hemoglobin typing using cellulose acetate electrophoresis in 2 Chaonam populations; 64 and 65 individuals inhabited in Tachatchai Tambol Maikaw Amphur Talang Phuket Province and Kaosirei Tambol Ratsada Ampher Muang Phuket Province were carried out. It was found that the incidences of Hb E heterozygotes are 6.25% and 10.76%, respectively and betaE globin gene frequencies f(betaE) are 0.031 and 0.054 respectively. BetaE globin gene associated with haplotypes were determined using PCR following by restriction enconuclease analysis. The most common haplotype are +----betaE+- or -+-++betaE+- which associated with FW 2 similar to the previous studies in Thais, Phutai, Chong, Laos Song and Kui population indicating the same origin of betaE globin gene. The other haplotype is -+-++betaE-+ which associated with FW 3 that are found commonly among Cambodian. In betaA gene we found many haplotypes, one is +----betaA+- which associated with FW 2, the other haplotypes are +----betaA-+ and -++-+betaA-+ which associated with FW 3. These haplotypes and frameworks associated with betaA globin gene which was commonly found among ethnic groups of Thailand. Deletion types and frequencies of alpha- globin gene were also examined in these chaonam populations. Only one type of alpha- globin gene deletion, rightward deletion type (-alpha3.7) was found. Rightward deletion type (-alpha3.7) determined by Southern blot. The frequencies of rightward deletion in Chaonam populations are 0.089.