Single cells of Holy mushroom (Ganoderma lucidum) strain MuG 003 and MUG 004 was induced for mutation. The single cells were produced by 6900 rpm homogenizing of 6 days old mycelia for 10 minutes and filtered through 50 mu pore size filter cloth. The ten percentage survival of the cells, exposed cells to ultraviolet light for 25 second, at 254 nm and at 22 cms in distance, were selected. The ten percentage survival of the cells, treated 125 ug/ml NTG for 30 minutes were also selected. Induced mutation by ultraviolet light provided one nystatin resistance mutant and two crystal violet resistance mutants from MUG 004. Induced mutation by NTG provided three nystatin resistance mutants and four crystal violet resistance mutants from MUG 003, and four nystatin resistance mutants and three crystal violet resistance mutants from MUG 004. The ultraviolet light inducing stains 003-UV-cv1 and 004-UV-ny3 produced the highest polysaccharide per DNA ratio. Strain 003-UV-cv1 had the ratio of 10.73 and 004-UV-ny3 had theratio of 6.48, while the wild type of MUG 003 and MUG 004 had the ratio of 5.72 and 3.85, respectively. The NTG inducing stains 003-NTG-ny3 and 004-NTG-cv1 produced the highest polysaccharide per DNA ratio. Strain 003-NTG-ny3 had the ratio of 6.96 and 004-NTG-cv1 had the ratio of 5.59. All resistant mutants morphology, the size of cell, the color of colony and the clamp connection, were not different from the wild type. But some resistant mutants had fluffy mycelium, which were different from the wild type. Mycelial growth rate of all mutants of MUG 003 were lower than that of the wild type. Mycelia growth rate of the mutant of MUG 004 were similar to that of the wild type.
