การศึกษาลักษณะแฮปโพลไทป์ของยีนบีตาอีโกลบินและยีนแอลฟาโกลบิน ในชนกลุ่มไทพวน จังหวัดลพบุรี เลย และอุดรธานี / อุรศรี สูยะศุนานนท์ = Studies of beta e-globin haplotypes and alpha-globin gene in Thai-Poun populations in Lopburi, Loei and Udon Thani provinces / Urasri Suyasunanont
Haemoglobin typing using cellulose acetate electrophoresis (TBE buffer, pH 8.6) in 3 Thai-poun populations; 89, 74 and 69 individuals inhabited in Amphur Banmee Lopburi province, Amphur Cheingkan Loei province and Amphur Banphu Udonthani province were carried out. It was found that the incidences of HbE are 16.85%, 41.85% and 11.50%, respectively and betaE-globin gene frequencies are 0.090, 0.230 and 0.065, respectively. The betaE-globin gene haplotype was determined by PCR following by restriction endonuclease analysis at 7 polymorphic sites. The most common haplotypes are -+-++betaE+- (43.33%) and +----betaE+- (40.00%) which associated with FW2 similar to the previous studies in Thais, Phutai and Laos Song. The rest 3 types are -+--+betaE++ +----betaE-+ and -++-+betaE+-. The betaE-globin gene haplotype was also determined. The most common 5'-haplotype is +---- (85.55%) which associated with 3 frameworks (FW3, FW2 and FW1). Types and frequencies of alpha-globin gene using Southern blot hybridization and PCR were also examined in these Thai-poun populations. Four types of alpha-globin gene were found. Gene frequencies of rightward deletion (-alpha3.7), leftward deletion (-alpha4.2), Southeast Asian deletion (--SEA) and triplicated alpha-globin (alpha alpha alpha) are 0.042, 0.005, 0.056 and 0.005, respectively.
