The aims of the present study are to produce n-3 fatty acids-lecithins and to evaluate their effects on growth, survival and low salinity stress of Penaeus monodon larvae. Lecithins extracted from Danish fish meal, Thai local fish meal and soybean shows different composition of fatty acids. Danish and local fish meal lecithin had higher EPA and DHA, whereas soybean lecithin had higher linoleic and alpha-linolenic acids. Phospholipid content in lecithins derived from soybean, Danish fish meal and local fish meal were 50, 25 and 20 g/100 g. Four diets; soybean lecithin-added diet (SAD), Danish fish meal lecithin-added diet (DAD), local fish meal lecithin-added diet (LAD) and free lecithin-added diet (control diet, CD) formulated from purified diets were used to feed shrimp larvae at different stages (zoea, mysis, and postlarvae). The amount of lecithin in lecithin added diets was 1.5% of the total ingredient. Shrimp larvae fed SAD showed the highest growth rate in both salinity 25 and 30 ppt. Shrimp larvae fed CD also showed the high growth rate without significant difference at 25 ppt. The shrimp larvae survival rate in all diets was not significant different. Regarding stress test, shrimp postlarvae fed all lecithin added diets showed more tolerance to low salinity compared to that of lecithin-free diet (CD). Moreover, the larvae fed LAD accumulated HUFA in tissue higher than the those fed other diets.