ผลของสารควบคุมการเจริญเติบโตในการเพาะเลี้ยงเนื้อเยื่อเปล้าน้อย Croton sublyratus Kurz / ประภาพรรณ ยังสุขยิ่ง = Effect of growth regulators on tissue culture of Croton sublyratus Kurz / Prapapun Youngsukying
Effect of growth regulators on tissue culture of Croton sublyratus Kurz was studied. The formation of shoot from shoot tip and lateral bud cultivated on modified Murashige and Skoog (MS) medium supplemented with cytokinins, BA and kinetin, at concentration of 0.5, 1.0, 5.0 and 10.0 mg/l and on growth regulator-free MS medium were determined. Only one shoot could be generated from shoot tip and lateral bud. Multiple shoots could be obtained when the stem segments were cultivated on MS medium containing BA at 1 mg/l in the dark for 7 weeks. Eighty-five percent of segments generated multiple shoots with the average of 6 shoots per segment. The shoot height of 0.5-2.0 cm was appropriated for transferring to the light. The segment between the 4th- 7th node from the tip was suitable for multiple shoots induction with April to June was the best harvesting time for the most effective shoot proliferation. The induction of root from these shoots by various growth regulators which were IAA, IBA or NAA at concentration of 0.1, 0.2, 0.5, 1.0, 3.0, 5.0 and 10.0 mg/l was also investigated. Supplementation of IAA at 1 mg/l in MS medium could induce root formation when cultivated in the dark for 2 weeks prior to transferring to the light while the other growth regulator did not induce root formation.
