ผลของสารสกัดจากใบต้นถอบแถบน้ำต่อการตั้งครรภ์ในหนูแรท / พิมพันธุ์ สายเพชร = Effects of crude extract of from leaves Derris trifoliata Lour. on pregnancy in rats / Pimphan Saiphet
The aim of this study was to investigate effects of alcoholic extract from Derris trifoliata Lour. leaves on implantation in rats. Doses of 150 300 and 600 mg/kg b.w. were orally administered to pregnant rats during day 1 to day 6 and during day 6 to day 11 of pregnancy. Laparotomy was performed on the 7th and 13th day of pregnancy. Implantation sites were observed and uterine tissue was sampling for the histological study. Gestational period number of litters weight and abnormality of offsprings were studied from pregnant rats of all groups that were reared until parturition. Results obtained showed that rats received the extract during day 1-6 of pregnancy exhibited lower numbers of implantation sites in all doses of study i.e. 8.30+-0.44 (150 mg) 7.20+-0.46 (300 mg.) and 4.77+-0.52 (600 mg.) compared to the control i.e. 11.10+-0.31. Lowering in numbers and weight of offsprings were presented in these treated group at the dose of 300 mg/kg b.w. and 600 mg/kg b.w. when compared to the control. Histological changes of uterus was studied. The enlargement and widen lumen with debris of blastocyst and desquamated endothelial lining cells were observed. There were hemorrhagic and thrombi in the lumen. Numerical increasing and enlargement of endometrial gland in stroma of mucosa were noticed. Wite blood cells infiltration were distributed in mesometrium triangle of myometrium layer. Rat received the extract the during D6-11 of pregnancy exhibited lower numbers of implantation sites in all doses i.e. 9.20+-0.44 (150 mg) 7.90+-0.56 (300 mg.) and 6.50+-0.45 (600 mg.) compared to the control i.e. 10.80+-0.38. Lowering in weight and numbers of offspring were present in all doses of orally administrated rats. Histological alteration of implanted uterus composed of the damaged of inner cellmass and trophoblast. Hemorrhagic and thrombi necrotic cells and inflamation with white blood cells infiltration were seen in the area of decidual basalis dacidual capsularis myometrium and uterine lumen. It is concluded that the leaves crude extract of Derris trifoliata is more effectively on implantation of pre-implantation period (D1-6) than during implantation period (D6-11) and in the manner of dose dependent effect.
