ผลของไพเพอรีนที่มีต่อปฏิกริยาที่เกี่ยวข้องกับพลังงานของไมโตคอนเดรีย ที่แยกจากตับหนูขาว / วันทนา เหรียญมงคล = The effect of piperine on energy-linked reactions of isolated rat liver mitochondria / Wantana Reanmongkol
Piperine is a member of the piperidine amide group of alkaloid found in various piper species of Piperaceae family. The chemical structure of piperine is trans 2, 4-pentadieneoic acid piperidide. The results on the effect of piperine on oxidative phosphorylation by rat liver mitochondria demonstrated that this process was inhibited by piperine. Both state 3 and DNP-stimulated respiration were depressed by piperine. Piperine also inhibited calcium-stimulated respiration by respiring mitochondria in the presence of glutamate + malate or succinate as respiratory substrates. Piperine diminished the oxidation of added NADH or succinate but had no effect on the oxidation of ascor- bate + TMPD by hypotonic-treated mitochondria. Piperine also activated ATPase in rat liver mitochondria but was less potent than DNP. Oligomycin and atractyloside only partially inhibited the piperine- induced ATPase activity. In addition, piperine inhibited calcium uptake by mitochondria and promoted the release of accumulated calcium. These findings strongly suggest that piperine interferes with oxidative phosphorylation and other energy-linked functions by retarding electron flow from coenzyme Q to cytochrome c in the mitochondrial respiratory chain. Piperine may act directly on ATPase complex to cause activation of this mitochondrial enzyme.
