The queens of Apiscerana Fabricius were produced by grafting larvae. The result showed that 92% of transplanted larvae were accepted and developed to be queens. The queens were inseminated with semen which were collected from drones by modified artificial insemination apparatus. The result showed that 68% of them survived after inseminating for 2 weeks and 30% were survived until they laid eggs and emerged to be workers. Egg laying activity of the queens was compared within 3 groups; 1) natural queens, 2) rearing queens with naturally mated, 3) rearing queens with artificially inseminated. The result showed that there was significant difference in each group (P < 0<05>. However, no significant difference was found in the amount of spermatozoa in the spermatheca between rearing queens with naturally mated and rearing queens with artificially in eminabed (P > 0.05).