Dried rhizomes of Euphorbia sessiliflora Roxb. Were finely crushed and extracted with hexane, giving a brown crude extract. The residue was further extracted with dichloromethane and methanol, giving a brown and a dark red crude extracts respectively. Fractionation of crude extracts by quick column chromatography and column chromatography led to the isolation of ten compounds. The structure of these compounds were established on the basis of physical properties, chemical properties and spectral evidences. Eight identified components were a mixture of long chain esters, a mixture of long chain alcohols (C28, C30), trans-triaconty1 4-hydroxy-3-methoxy cinnamate, Jolkinolide A, a mixture of β-sitosterol, stigmasterol and cholesterol, Jolkinolide B, a mixture of β-sitosteryl 1-3-0-β-D-glicopyranoside and stigmasteryl 1-3-0- β -D-glucopyranoside, Lanosterol. The other two unidentified components were compound were compound 9 (m.p. 191-192 °C, and compound 10 (m.p. 248-251°C)
