The molecular cloning of genes - general procedures -- Bacteriophage λ as a vector in recombinant DNA research-advantages and limitations -- Cloning cDNA sequences: a general technique for propagating eukaryotic gene sequences in bacterial cells -- Plasmid cloning vectors -- In vivo formation of recombinant DNA molecules by IS-elements and transposons -- Development of bacillus subtilis as a cloning system -- Potential application of molecular cloning to genetic engineering -- Social and political issues of genetic engineering -- Microbial genetic engineering -- Microbial genetic engineering by natural plasmid transfer - some representative benefits and biohazards -- index

1. Genetic engineering
2. Genetic intervention
3. Cloning
4. Molecular
5. DNA
6. Recombinant
7. Genetic Vectors