The purpose of this research is to study gender and homosexuality in Thai films as well as analyze narrative methods that convey messages of gender and homosexuality using the following concepts: gender, sexuality, homosexual men, semiotics, narrative films, and social construction of reality for analytical guidelines. The study uses 25 Thai films with content and characters revolving around homosexual men as samples for case study. The research findings reveal that in Thai films, the most frequent incidence of homosexual men is found in character cast as comedians and the least frequent as average people. The gender, established by the film’s development of social and cultural composition, has a part in determining the gender meaning of homosexual men, affecting the role, duty, and rights of the character cast as a homosexual men in the film. The researcher has determined that in the films, there were 4 types of sexuality in characters cast as homosexual men: queer, gay, bisexual and ambiguous. In the films, each type begins with the establishment of the character’s sexuality as a homosexual men expressing sexual identity, sexual desire, sexual actions, sexual satisfaction and sexual preference. In addition, the characteristics of the homosexual men’s sexuality expression are prescribed by a social system of thoughts and beliefs.The research findings show that various narrative methods to convey messages of gender and sexuality regarding homosexual men in Thai films are used through the narrative structure, binary opposition, and code.