New rice varieties; RD 21, RD 23, RD 25 and RD 27, were investigated to determine their nutritional values compared with two parent varieties; Khao Dawk Mali 105 and Khao Ta Hang 17. The results of Chemical analysis of those milled and dehulled rice sample revealed that 12-13% of water existed in four new varieties of rice samples. Fat content was found about 1-25 indehulled rice samples of RD 21, RD 23, RD 25 and RD 27, but only 0.2% fat was found in those milled rice samples. Khao Dawk Mali 105 and Khao Ta Hang 17 contained 2.3% fat in their dehulled samples and only 0.4% fat found in milled samples. All of tested milled samples contained about 77-78% of carbohydrate and about 73-74% of carbohydrate was found in dehulled samples. Protein content was about 9-10% in both milled and dehulled rice samples tested. About 0.7-0.9% of crude fiber was found in dehulled rice and only about 0.1-0.2% of crude fiber remained in milled samples. Ash content was about 1% in dehulled and 0.2-0.6% in milled rice samples,respectively. Vitamin B[subscript 1], Vitamin B[subscript 2] and niacin levels were higher in dehulled rice than milled rice samples. Mineral contents such as calcium, iron, phosphorus, sodium, potassium, magnesium, copper, zinc, manganese and alimunium were determined. Dehulled rice samples contained higher level of calcium, phosphorus, potassium, magnesium and manganese than their milled samples. However, the levels of sodium, zinc and aluminium were not much different in milled and dehulled rice samples. Trace of copper was found in rice. Amino acid composition in rice protein was investigated. Each variety of tested rice protein contained all kinds of essential and non-essential amino acids. Each sample seemed to contain a similar profile of their amino acid contents. Amino acid scores of lysine and isoleucine were rather low. Chemical score of rice protein was about 60-70. Four new varieties of tested rice samples seem to have good nutritional values similar to two parent varieties of tested rice samples.