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TitleArthritis Research [electronic resource] : Methods and Protocols Volume 2
Author edited by Andrew P. Cope
ImprintTotowa, NJ : Humana Press, 2007
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Descript XXII, 432 p. 79 illus., 6 illus. in color. online resource


Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking. In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise. Arthritis Research: Methods and Protocols will be a valuable tool for basic research investigators, clinician scientists, pathologists, immunologists, and biochemists looking to stay current in their fields


Immunobiology -- Phenotypic Analysis of B-Cells and Plasma Cells -- Detection of Antigen Specific B-Cells in Tissues -- Single Cell Analysis of Synovial Tissue B-Cells -- Tracking Antigen Specific CD4+ T-Cells With Soluble MHC Molecules -- Analysis of Antigen Reactive T-Cells -- Identification and Manipulation of Antigen Specific T-Cells with Artificial Antigen Presenting Cells -- Analysis of Th1/Th2 T-Cell Subsets -- Analysis of the T-Cell Receptor Repertoire of Synovial T-Cells -- The Assessment of T-Cell Apoptosis in Synovial Fluid -- Assays of T-Cell Contact Dependent Monocyte-Macrophage Functions -- Phenotypic and Functional Analysis of Synovial Natural Killer Cells -- Identification and Isolation of Synovial Dendritic Cells -- Animal Models of Arthritis -- The Use of Animal Models for Rheumatoid Arthritis -- Collagen-Induced Arthritis in Mice -- Collagen-Induced Arthristis in Rats -- Collagen Antibody Induced Arthritis -- Arthritis Induced with Minor Cartilage Proteins -- Murine Antigen-Induced Arthritis -- Pristane-Induced Arthritis in the Rat -- The K/BxN Mouse Model of Inflammatory Arthritis -- Analysis of Arthritic Lesions in the Del1 Mouse -- Application of New Technologies to Define Novel Therapeutic Targets -- Gene Expression Profiling in Rheumatology -- Differential Display Reverse Transcription-Polymerase Chain Reaction to Identify Novel Biomolecules in Arthritis Research -- Two-Dimensional Electrophoresis of Proteins Secreted from Articular Cartilage -- Mapping Lymphocyte Plasma Membrane Proteins -- In Vivo Phage Display Selection in the Human/SCID Mouse Chimera Model for Defining Synovial Specific Determinants -- Adenoviral Targeting of Signal Transduction Pathways in Synovial Cell Cultures

Medicine Human genetics Orthopedics Cell biology Medicine & Public Health Orthopedics Human Genetics Cell Biology


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