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TitleBiotechnology of Plant Secondary Metabolism [electronic resource] : Methods and Protocols
Author edited by Arthur Germano Fett-Neto
ImprintNew York, NY : Springer New York : Imprint: Humana Press, 2016
Edition 1st ed. 2016
Connect tohttp://dx.doi.org/10.1007/978-1-4939-3393-8
Descript XI, 180 p. 37 illus., 15 illus. in color. online resource

SUMMARY

This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry


CONTENT

Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal -- Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction -- A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines -- Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves -- From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate : Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica -- Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli -- Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala -- Production of Aromatic Plant Terpenoids in Recombinant Bakeŕs Yeast -- Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC) -- A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes -- Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters -- Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants -- Protoplast Transformation as a Plant-Transferable Transient Expression System -- Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System -- Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs


Life sciences Plant science Botany Life Sciences Plant Sciences



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