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TitleBiosensors with Fiberoptics [electronic resource] / edited by Donald L. Wise, Lemuel B. Wingard
ImprintTotowa, NJ : Humana Press : Imprint: Humana Press, 1991
Connect tohttp://dx.doi.org/10.1007/978-1-4612-0483-1
Descript XIV, 370 p. online resource

SUMMARY

Focusing on the rapidly increasing interaction between biotechnology and advanced fiberoptics/electronics, Biosensors with Fiberoptics emphasizes the three major phases of the developmental process from concept to marketplace: research, development, and applications. With contributions by leading experts directly involved in key areas of this exciting, fast-paced field, the book describes novel biosensor technologies and such current and potential applications as: chemical sensing โ{128}ข fluorescent labels โ{128}ข evanescent-wave biosensors and immunosensors โ{128}ข clinical applications โ{128}ข immunoassay kinetics โ{128}ข luminescence โ{128}ข monitoring of environmental toxins. Researchers and clinicians in a broad spectrum of disciplines will find Wise and Wingard's Biosensors with FIBEROPTICS an extremely practical, up-to-date reference text


CONTENT

Concepts, Biological Components, and Scope of Biosensors -- 1. Concept of a Biosensor -- 2. Biological Components -- 3. Transducers -- 4. Potential Applications -- References -- Chemical Sensing with Fiberoptic Devices -- 1. Introduction -- 2. Classification -- 3. Instrumentation -- 4. Analytical Characteristics -- 5. pH Sensors -- 6. Sensors for Anions -- 7. Sensors for Cations -- 8. Sensors for Gases -- 9. Sensors for Biochemicals -- 10. Sensors for Organic Compounds -- 11. Miscellaneous Sensors -- 12. Conclusions -- References -- Fluorescent Labels -- 1. Introduction -- 2. Fluorescence Excitation and Detection -- 3. Chemically Reactive Groups -- 4. Conclusions -- References -- Chemistry and Technology of Evanescent Wave Biosensors -- 1. Introduction -- 2. Why Fiberoptics? -- 3. Fiberoptic Fundamentals -- 4. Configurations for Fiberoptic Biosensors -- 5. Biochemical Architecture of Biosensors -- 6. Approaches for Improved Fiberoptic Biosensors -- 7. Summary -- Acknowledgments -- References -- Optical Characteristics of Fiberoptic Evanescent Wave Sensors Theory and Experiment -- 1. Introduction -- 2. Theory of Evanescent Wave Excitation and Detection of Fluorescence -- 3. Results Calculated from Theory -- 4. Results Based on Experiments -- 5. Summary and Conclusions -- 6. Symbols -- Acknowledgments -- References -- Evanescent Wave Immunosensors for Clinical Diagnostics -- 1. Introduction -- 2. Materials and Methods -- 3. Characterization of the Fiber as a Solid-Phase Surface-Isotopic Model -- 4, Fiberoptic Evanescent Wave Immunoassay (EWS) -- 5. Summary and Discussion -- References -- Instrumentation for Cylindrical Waveguide Evanescent Fluorosensors -- 1. Introduction -- 2. Evanescent Waves -- 3. Importance of Numerical Aperture -- 4. Why Cylindrical Waveguides? -- 5. Optical System -- 6. Excitation System -- 7. Detection System -- 8. Filters -- 9. Optical Background -- 10. Sensitivity -- 11. Multiplex Operation -- 12. Applications -- Acknowledgments -- References -- Immunoassay Kinetics at Continuous Surfaces -- 1. Introduction -- 2. Abbreviations -- 3. The Continuous Surface -- 4. Effects of Diffusion at Continuous Surfaces -- 5. Optical Techniques Applied to Immunoassays at a Continuous Surface -- 6. Discussion and Conclusions -- Acknowledgment -- References -- Luminescence in Biosensor Design -- 1. Introduction -- 2. Bioluminescent Reactions -- 3. Immobilized Enzymes for Bioluminescent Analysis 2% -- 5. Bio-and Chemiluminescence Analysis with Fiberoptic Sensors -- 6. Conclusion and Future Trends -- References -- In Vivo Applications of Fiberoptic Chemical Sensors -- 1. Introduction -- 2. Critical Issues and Design Criteria -- 3. Oxygen Sensors -- 4. pH Sensors -- 5. Carbon Dioxide Sensors -- 6. Conclusions -- References


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