การศึกษาเปรียบเทียบผลของกลาสไอโอโนเมอร์ซีเมนต์ชนิดดั้งเดิม และกลาสไอโอโนเมอร์ซีเมนต์ชนิดดัดแปลงด้วยเรซินต่อเซลล์เอ็นยึดปริทันต์ของมนุษย์ / นิรันดร โถทองคำ = A comparative study of the effects of conventional glass ionomer cement and resin modified glass ionomer cement on human periodontal ligament cells / Nirundorn Thotongkome
The objective of this study was to investigate effects of conventional glass ionomer cement (CGIC) compared with resin modified glass ionomer cement (RMGIC) on human periodontal ligament cells. The effects of the direct contact of human periodontal ligament cells on these material surfaces after 1, 3 and 5 days incubation were studied. The study focused on the growth rate, morphological appearances using scanning electron microscope (SEM), fibronection expression and alkaline phosphatase activity (ALP activity). The results showed that freshly mixed of both materials had highly cytotoxicity to human periodontal ligament cells but cytotoxicity level decreased constantly after 7 days of immersion materials in culture medium. Human PDL cells could grow on both material surfaces. On days 3, there was no statistical significant difference on growth rate amongst the control group and all experimental groups. However, on day 5, the growth rate of the RMGIC group was statistically greater than the control group (p < 0.05) but it was not statistically different from the CGIC group. Meanwhile, CGIC group had a higher growth rate than the control group but there was no statistical difference. The result from SEM study demonstrated that PDL cells could attach to surfaces of both materials with normal appearance. Western blot analysis showed that fibronectin level from cells in the RMGIC group was greater than both control and CGIC groups. Finally, the ALP activity of both RMGIC and CGIC groups were not statistically different but were statistically lower than that of the control group (p < 0.05). It was concluded that PDL cells could normally grow and proliferate on both material surfaces. Growth rate of PDL cells was not inhibited by both materials. The RMGIC had more positive effects on PDL cells than the CGIC.